@Article{phyton.2020.09259,
AUTHOR = {Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li},
TITLE = {Reference Gene Selection for Quantitative Real-Time PCR Analyses of <i>Acer palmatum</i> under Abiotic Stress},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {2},
PAGES = {385--403},
URL = {http://www.techscience.com/phyton/v89n2/38679},
ISSN = {1851-5657},
ABSTRACT = {Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important
for obtaining authentic normalized data. <i>Acer palmatum</i> is an important colorful
leaf ornamental tree species, and reference genes suitable for normalization of the
qRT-PCR data obtained from this species have not been investigated. In this
study, the expression stability of ten candidate reference genes, namely, <i>Actin3</i>,
<i>Actin6</i>, <i>Actin9</i>, <i>EF1α</i>, <i>PP2A</i>, <i>SAMDC</i>, <i>TIP41</i>, <i>TUBα</i>, <i>TUBβ</i> and <i>UBQ10</i>, in
two distinct tissues (leaves and roots) of <i>A. palmatum</i> under four different abiotic
stress conditions (cold, heat, salt and drought) were investigated and assessed
using three statistical methods (GeNorm, NormFinder and BestKeeper). The combinations of reference genes that showed stability in the different stressed samples
differed. Specifically, <i>Actin6</i>, <i>UBQ10</i> and <i>Actin9</i> were the most stable reference
genes in all the samples, and <i>Actin3</i> and <i>Actin6</i> wexre stably expressed in coldstressed leaves and roots. <i>Actin3</i>, <i>Actin6</i> and <i>UBQ10</i> were identified as an appropriate combination of reference genes for the analysis of heat-stressed leaves and
roots, whereas the combination of <i>Actin9</i>, <i>UBQ10</i> and <i>Actin6</i> was deemed the
most suitable for the analysis of salt-stressed leaves and roots. Similarly, <i>Actin6</i>
and <i>UBQ10</i> exhibited stable expression in drought-stressed leaves and roots.
Furthermore, the expression levels of CBF, <i>Cu/Zn-SOD</i> and <i>HsfA1</i> were estimated
to determine the reliability of the reference genes assessed in this study. This
study revealed stable reference genes in <i>A. palmatum</i> that might be used for
the normalization of qRT-PCR data obtained under various abiotic stresses.},
DOI = {10.32604/phyton.2020.09259}
}