@Article{phyton.2020.09769,
AUTHOR = {Gloria Viviana Cerrillo-Rojas, Mariana Tiscareño-Andrade, Ana Erika Ochoa-Alfaro, Eugenio Pérez-Molphe Balch, Ruth Elena Soria-Guerra, José Francisco Morales-Domínguez},
TITLE = {<i>In Vitro</i> Propagation, Isolation and Expression Studies of <i>Suaeda edulis</i> Genes Involved in the Osmoprotectants Biosynthesis},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {3},
PAGES = {715--726},
URL = {http://www.techscience.com/phyton/v89n3/39408},
ISSN = {1851-5657},
ABSTRACT = {Halophytes are an excellent choice for the study of genes conferring
salt tolerance to salt-sensitive plants and, they are suitable for reclamation and
remediation of saline soil. We develop an <i>in vitro</i> plant propagation protocol
and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis.
Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and
combinations of plant growth regulators. The highest number of multiple shoots
was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ± 06 shoots per explant. The identification and expression
analysis of genes involved in glycine betaine (GB) biosynthesis were S-adenosylmethionine synthetase (<i>SAMS</i>), choline monooxygenase (<i>CMO</i>) and betaine aldehyde dehydrogenase (<i>BADH</i>), and for proline (Pro) was pyrroline 5-carboxylate
synthetase (<i>P5CS</i>). These sequences shared 90–95% of identity with others plant
homologous in public databases. The amino acids sequence analysis showed that
all these peptides contain some of the conserved motifs of those kinds of
enzymes. The qRT-PCR analysis revealed a higher expression of <i>SeBADH</i>,
<i>SeCMO</i>, and, <i>SeP5CS</i> genes in the roots and leaves from plants collected in
the field in contrast with from <i>in vitro</i> plants. However, the expression level of
<i>SeSAMS</i> was higher only in the leaves of plants collected in the field when compared to those cultivated <i>in vitro</i>.},
DOI = {10.32604/phyton.2020.09769}
}