TY - EJOU
AU - Cerrillo-Rojas, Gloria Viviana
AU - Tiscareño-Andrade, Mariana
AU - Ochoa-Alfaro, Ana Erika
AU - Balch, Eugenio Pérez-Molphe
AU - Soria-Guerra, Ruth Elena
AU - Morales-Domínguez, José Francisco
TI - In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis
T2 - Phyton-International Journal of Experimental Botany
PY - 2020
VL - 89
IS - 3
SN - 1851-5657
AB - Halophytes are an excellent choice for the study of genes conferring
salt tolerance to salt-sensitive plants and, they are suitable for reclamation and
remediation of saline soil. We develop an in vitro plant propagation protocol
and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis.
Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and
combinations of plant growth regulators. The highest number of multiple shoots
was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ± 06 shoots per explant. The identification and expression
analysis of genes involved in glycine betaine (GB) biosynthesis were S-adenosylmethionine synthetase (SAMS), choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), and for proline (Pro) was pyrroline 5-carboxylate
synthetase (P5CS). These sequences shared 90–95% of identity with others plant
homologous in public databases. The amino acids sequence analysis showed that
all these peptides contain some of the conserved motifs of those kinds of
enzymes. The qRT-PCR analysis revealed a higher expression of SeBADH,
SeCMO, and, SeP5CS genes in the roots and leaves from plants collected in
the field in contrast with from in vitro plants. However, the expression level of
SeSAMS was higher only in the leaves of plants collected in the field when compared to those cultivated in vitro.
KW - Axillary buds; halophyte; proline; glycine betaine; salt-resistant
DO - 10.32604/phyton.2020.09769