@Article{phyton.2020.011728,
AUTHOR = {Longhua Zhou, Ting He, Guimei Guo, Jing Li, Ruiju Lu, Yingbo Li, Chenghong Liu},
TITLE = {Genome-Wide Identification of Candidate Genes Associated with <i>β</i>-glucan Traits in a Hulled and Hulless Barley (<i>Hordeum vulgare</i> L.) Population},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {4},
PAGES = {987--997},
URL = {http://www.techscience.com/phyton/v89n4/40525},
ISSN = {1851-5657},
ABSTRACT = {Barley grain is a valuable source of <i>β</i>-glucan, which is an important
component of dietary fiber with significant human health benefits. Although the
genetic basis of <i>β</i>-glucan biosynthesis has been widely studied, a genome-wide
association study (GWAS) is still required for a scan of the candidate genes
related to the complex quantitative trait based on the high-quality barley reference
genome. In this study, a GWAS was conducted using a population composed of
87 barley landraces (39 hulled and 48 hulless, <i>β</i>-glucan from 2.07% to 6.56%)
with 191,098 nucleotide polymorphisms (SNPs) markers to cover the chromosomes with the highest density. The population was divided into four sub-populations (POP1~POP4), and the <i>β</i>-glucan content in POP2 was significantly higher
than that in other groups, in which most of the hulless barley landraces are from
Qinghai-Tibet Plateau in China. Among seven SNP markers identified by GWAS,
two (SNP2 and SNP3) of them showed positive correlation to <i>β</i>-glucan trait and
the remaining five (SNP1, SNP4, SNP5, SNP6 and SNP7) showed the negative
relationship. Two candidate genes linked to SNP7, <i>HORVU7Hr1G000320</i> and
<i>HORVU7Hr1G000040</i>, belong to the nucleotide triphosphate hydrolase superfamily which is probable to affect the activities of <i>β</i>-glucan synthase. Another
candidate gene associated with SNP1, <i>HORVU1Hr1G000010</i>, is possibly
involved in sugar response. In conclusion, our results provide new insights into
the genetic basis of <i>β</i>-glucan accumulation in barley grains, and the discovery of
new SNP markers distributed in each chromosome and the associated candidate genes
will be valuable for the breeding of functional barley varieties with high <i>β</i>-glucan.},
DOI = {10.32604/phyton.2020.011728}
}