@Article{phyton.2020.011184,
AUTHOR = {Meiling Tang, Yuhe Li, Yahui Chen, Lei Han, Hongxia Zhang, Zhizhong Song},
TITLE = {Characterization and Expression of Ammonium Transporter in Peach (Prunus persica) and Regulation Analysis in Response to External Ammonium Supply},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {89},
YEAR = {2020},
NUMBER = {4},
PAGES = {925--941},
URL = {http://www.techscience.com/phyton/v89n4/40530},
ISSN = {1851-5657},
ABSTRACT = {As the preferred nitrogen (N) source, ammonium (NH4+
) contributes to
plant growth and development and fruit quality. In plants, NH4+ uptake is facilitated by a family of NH4+ transporters (AMT). However, the molecular mechanisms and functional characteristics of the AMT genes in peach have not been
mentioned yet. In this present study, excess NH4+ stress severely hindered shoot
growth and root elongation, accompanied with reduced mineral accumulation,
decreased leaf chlorophyll concentration, and stunned photosynthetic performance. In addition, we identified 14 putative AMT genes in peach (PpeAMT).
Expression analysis showed that PpeAMT genes were differently expressed in
peach leaves, stems and roots, and were distinctly regulated by external NH4+
supplies. Putative cis-elements involved in abiotic stress adaption, Ca2+ response,
light and circadian rhythms regulation, and seed development were observed in
the promoters of the PpeAMT family genes. Phosphorylation analysis of residues
within the C-terminal of PpeAMT proteins revealed many conserved phosphorylation
residues in both the AMT1 and AMT2 subfamily members, which could potentially
play roles in controlling the NH4+ transport activities. This study provides gene
resources to study the biological function of AMT proteins in peach, and reveals
molecular basis for NH4+ uptake and N nutrition mechanisms of fruit trees.},
DOI = {10.32604/phyton.2020.011184}
}