@Article{phyton.2020.013933,
AUTHOR = {Yan Li, Renquan Huang, Jianrong Li, Xiaozhen Huang, Xiaofang Zeng, Degang Zhao},
TITLE = {<i>DWARF</i> and <i>SMALL SEED1</i>, a Novel Allele of <i>OsDWARF</i>, Controls Rice Plant Architecture, Seed Size, and Chlorophyll Biosynthesis},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {1},
PAGES = {111--127},
URL = {http://www.techscience.com/phyton/v90n1/40608},
ISSN = {1851-5657},
ABSTRACT = {Plant architecture is a vital agronomic trait to control yield in rice (<i>Oryza sativa</i> L.). A <i>dwarf and small seed 1</i> (<i>dss1</i>) mutant were obtained from the ethyl methanesulfonate (EMS) mutagenized progeny of a Guizhou glutinous landrace cultivar, Lipingzabianhe. The <i>dss1</i> mutant displayed phenotypes similar to those of brassinosteroid (BR) deficient mutants, such as dwarfing, dark green and rugose erect leaves, small seeds, and loner neck internode panicles with primary branching. In our previous study, the underlying <i>DSS1</i> gene was isolated, a novel allele of <i>OsDWARF</i> (<i>OsBR6ox</i>) that encodes a cytochrome P450 protein involved in the BR biosynthetic pathway by MutMap technology. In this work, we confirmed that a Thr335Ile amino acid substitution residing in DSS1/OsDWARF was responsible for the dwarf, panicle architecture, and small seed phenotypes in the <i>dss1</i> mutants by genetic transformation experiments. The overexpression of <i>OsDWARF</i> in the <i>dss1</i> mutant background could not only recover <i>dss1</i> to the normal plant height and panicle architecture but also rescued normal leaf angles, seed size, and leaf color. Thus, the specific mutation in DSS1/OsDWARF influenced plant architecture, seed size, and chlorophyll biosynthesis.},
DOI = {10.32604/phyton.2020.013933}
}