@Article{phyton.2020.011545,
AUTHOR = {Yinjie Wang, Yongxia Zhang, Qingquan Liu, Liangqin Liu, Suzhen Huang, Haiyan Yuan},
TITLE = {Reference Gene Selection for qRT-PCR Normalization in <i>Iris germanica</i> L.},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {1},
PAGES = {277--290},
URL = {http://www.techscience.com/phyton/v90n1/40611},
ISSN = {1851-5657},
ABSTRACT = {Quantitative real-time PCR (qPCR) is an effective and widely used
method to analyze expression patterns of target genes. Selection of stable reference genes is a prerequisite for accurate normalization of target gene expression
by qRT-PCR. In <i>Iris germanica</i> L., no studies have yet been published regarding
the evaluation of potential reference genes. In this study, nine candidate reference
genes were assessed at different flower developmental stages and in different tissues by four different algorithms (GeNorm, NormFinder, BestKeeper, and RefFinder). The results revealed that <i>ACT11</i> (Actin 11) and <i>EF1α</i> (Elongation factor
1 alpha) were the most stable reference genes in different tissues, whereas TUA
(Tubulin alpha) and <i>UBC9</i> (Ubiquitin-protein ligase 9) were the most stable ones
in different flower developmental stages. <i>UBC9</i> and <i>ACT11</i> were the most stable
reference genes in all of the tested samples, while the <i>SAMDC</i> (S-Adenosylmethionine decarboxylase) showed the least stability. Finally, to validate the suitability of the selected reference genes, the relative expression level of <i>IgTPS</i>
(beta-caryophyllene synthase) was assessed and highlighted the importance of
suitable reference gene selection. This work constitutes the first systematic evaluation of potential reference genes in <i>I. germanica</i> and provides guidelines for
future research on gene function and molecular mechanisms on <i>I. germanica</i>
and related species.},
DOI = {10.32604/phyton.2020.011545}
}