@Article{phyton.2021.012928,
AUTHOR = {Pengtao Li, Quanwei Lu, Xianghui Xiao, Rui Yang, Xixi Duan},
TITLE = {Dynamic Expression Analysis and Introgressive Gene Identification of Fiber Length Using Chromosome Segment Substitution Lines from <i>G. hirsutum</i> × <i>G. barbadense</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {1},
PAGES = {129--144},
URL = {http://www.techscience.com/phyton/v90n1/40616},
ISSN = {1851-5657},
ABSTRACT = {Fiber length is a critical trait that principally determines cotton spinning
quality, while Upland cotton as the most widely cultivated Gossypium species
around the world subjects to the relatively ordinary fiber performance. Chromosome segment substitution lines (CSSLs) have been introduced in cotton breeding
to take full advantages of superior fiber quality and high yield from Sea Island and
Upland cotton, respectively, which serve as ideal materials for elucidating the
genetic mechanism of complex quantitative traits. Here, three CSSLs derived
from CCRI45 (<i>G. hirsutum</i>) × Hai1 (<i>G. barbadense</i>), two superior (MBI7561 and
MBI7747) and one (MBI7285) with ordinary fiber-quality, were subjected to transcriptome sequencing during fiber elongation together with their recurrent parent
CCRI45, and 471.425 million clean reads were obtained with 91.47% average
Q30 and 45.23% mean GC content. In total, 5,673 differentially expressed genes
(DEGs) were identified from multi-sample comparisons, which were mainly
involved in the oxidation-reduction process, protein phosphorylation, regulation
of transcription, DNA template, and carbohydrate metabolic process. Eight temporal expression patterns were monitored on the DEGs of different lines, of which
the significantly enriched profile revealed higher similarities between two superior
CSSLs or the ordinary CSSL and CCRI45 with respect to fiber performance.
Based on the intersection between the predicted introgressive genes from RNAseq data and the published gene information from the G. barbadense genome,
1,535 introgressive genes were identified in three CSSLs. Further analysis of
the three common introgressive sections in superior CSSLs revealed eight candidate genes that were identified to be involved in fiber development, namely,
O-fucosyltransferase family protein (<i>GB_A02G0240</i>), glutamine synthetase 2
(<i>GB_A02G0272</i>), Ankyrin repeat family protein (<i>GB_A02G0264</i>), beta-6 tubulin
(<i>GB_D03G1742</i>), WRKY DNA-binding protein 2 (<i>GB_D03G1655</i>), quinolinate
synthase (<i>GB_D07G0623</i>), nuclear factor Y, subunit B13 (<i>GB_D07G0631</i>), and
leucine-rich repeat transmembrane protein kinase (<i>GB_D07G0797</i>). Our results provide novel insights into the mechanism underlying fiber formation and lay a
solid foundation for further high-efficiency determination of candidate genes by
combining RNA-seq data and pivotal chromosome regions.},
DOI = {10.32604/phyton.2021.012928}
}