@Article{phyton.2021.013223,
AUTHOR = {Anna Stepanova, Maria Malunova, Svetlana Salamaikina, Renat Selimov, Aleksandra Solov’eva},
TITLE = {Establishment of <i>Rhodiola quadrifida</i> Hairy Roots and Callus Culture to Produce Bioactive Compounds},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {2},
PAGES = {543--552},
URL = {http://www.techscience.com/phyton/v90n2/41466},
ISSN = {1851-5657},
ABSTRACT = {<i>Rhodiola quadrifida</i> is a rare mountain medicinal plant whose root
extracts are used in traditional Chinese medicine as a hemostatic, antitussive,
and tonic in the treatment of gynecological diseases. The aim of the study was
to obtain <i>R. quadrifida</i> cultures at different degrees of differentiation <i>in vitro</i>
and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of <i>Agrobacterium rhizogenes</i> strain A4. The presence of the rolB and
rolC genes was proven by polymerase chain reaction. The obtained roots were
cultivated in Murashige-Skoog medium (MS). Calluses were obtained from the
hairy roots in MS medium with the addition of hormones: 3 mg/L 2,4 D and
0.5 mg/L BAP. The presence of the main secondary metabolites of <i>R. quadrifida</i>,
salidroside and rosavin, in calluses and salidroside in hairy roots by HPLC/MS
was confirmed. The content of salidroside in callus culture was significantly
higher than in hairy roots, 0.158 and 0.047%, respectively. The content of rosavin
in callus culture was 0.07%. The content of rosavin and salidroside in callus culture
was close to the level of these substances in the rhizomes of <i>R. quadrifida</i> plants
growing <i>in vivo</i>, making this culture promising for its possible biotechnological use.},
DOI = {10.32604/phyton.2021.013223}
}