@Article{phyton.2021.014191,
AUTHOR = {Jiaji Zhang, Xiaofei Long, Yuhao Weng, Tielong Cheng, Jisen Shi, Jinhui Chen},
TITLE = {Efficient Evergreen Plant Regeneration of <i>Cinnamomum japonicum</i> Sieb. through <i>in vitro</i> Organogenesis},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {2},
PAGES = {571--582},
URL = {http://www.techscience.com/phyton/v90n2/41467},
ISSN = {1851-5657},
ABSTRACT = {<i>Cinnamomum japonicum</i> Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study,
we successfully developed a large-scale micropropagation protocol for <i>C. japonicum</i> for the first time. Sterilized shoots were excised and used as explants for
shoot induction on several basal media, supplemented with different concentrations
of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N<sup>6</sup>
-Benzyladenine
(6-benzylaminopurine) (BA), <i>α-naphthaleneacetic</i> acid (NAA) and Gibberellic acid
(GA<sub>3</sub>). After comparison, the most efficient medium for shoot regeneration
was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L<sup>–1</sup> BA,
0.05 mg L<sup>–1</sup> NAA and 0.2 mg L<sup>–1</sup> GA<sub>3</sub>, which resulted in an average number of
induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d, respectively. Then, elongated adventitious shoots were transferred to induce roots.
86.7% of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg
L<sup>–1</sup> NAA and 0.1 mg L<sup>–1</sup> BA. The earliest rooting time observed was after 21 d
and the average root length was up to 3.3 cm after 28 d. Our study shows that
<i>C. japonicum</i> can be successfully regenerated through <i>de novo</i> organogenesis,
which lays a foundation for future transformation research on this tree.},
DOI = {10.32604/phyton.2021.014191}
}