@Article{phyton.2021.013912,
AUTHOR = {Kuipeng Li, Hua Han, Yunhui Xie, Xiaomei Sun},
TITLE = {Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {2},
PAGES = {583--593},
URL = {http://www.techscience.com/phyton/v90n2/41475},
ISSN = {1851-5657},
ABSTRACT = {Optimization of <i>in vitro</i> adventitious root induction contributes to the development of a large-scale production
system of hybrid clone seedlings of larch (<i>Larix</i> spp.). We used orthogonal testing to investigate the factors that
affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu),
cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that
the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average
number of roots; and Glu on survival rate were all significant (<i>p</i> < 0.05) when compared to the mock treatments.
Among all of the 45 treatments, the highest rooting rate was 90%, the highest survival rate was 100%, and the
highest average root number was 11.8. The theoretically optimal procedure of in vitro root induction of hybrid
larch shoots is shoot induction on L9 modified medium (pH 6.7) containing 2 mg.L<sup>–1</sup> naphthalene acetic acid,
0.25 mg.L<sup>–1</sup> IBA, 1/3 macroelements, 7.5 g.L<sup>–1</sup> sucrose, and 5 mg.L<sup>–1</sup> Glu for 8 days, and then root induction
on L9 modified medium without auxin.},
DOI = {10.32604/phyton.2021.013912}
}