@Article{phyton.2021.016314,
AUTHOR = {Shiyang Li, Xue-Ao Liu, Lizi Zhao, Huiqing Huang, Bei Li, Zhizhong Song, Meixia Liang, Hongxia Zhang, Limin Wang, Shenglin Zhou},
TITLE = {Overexpression of <i>IbSINA5</i> Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {3},
PAGES = {761--772},
URL = {http://www.techscience.com/phyton/v90n3/41920},
ISSN = {1851-5657},
ABSTRACT = {Seven <i>in absentia</i> (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a <i>seven in absentia</i> gene <i>IbSINA5</i> was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that <i>IbSINA5</i> was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of <i>IbSINA5</i> in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of <i>IbCOR</i> (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H<sub>2</sub>O<sub>2</sub> accumulation in the leaves of transgenic plants. Furthermore, transient expression of <i>IbCBF3</i>, a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both <i>IbSINA5</i> and <i>IbCOR</i> genes. Our results suggest that <i>IbSINA5</i> could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance.},
DOI = {10.32604/phyton.2021.016314}
}