TY  - EJOU
AU  - Wei, Gang 
AU  - Yang, Hongxia 
AU  - Wu, Jingwen 
AU  - Xiong, Zixian 
AU  - Wang, Nan 

TI  - Phenotype Analysis and Fine Mapping of the Male Sterile Mutant <i>ms10</i> in Rice (<i>Oryza sativa</i> L.)
T2  - Phyton-International Journal of Experimental Botany

PY  - 2021
VL  - 90
IS  - 3
SN  - 1851-5657

AB  - There is a positive correlation between fertility and yield, and the decrease of fertility is bound to a greatly reduced
crop yield. Male sterile mutants can be used in hybrid rice. Therefore, rice male sterility has an important value in
research and application, and the study of related mutants is also very vital. The mutant <i>ms10</i> (<i>male sterile 10</i>)
reported in this study was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B.
There was no significant difference between the <i>ms10</i> and wild type in the vegetative growth stage. However, in
the reproductive growth stage, <i>ms10</i> showed that the plant became shorter, the anther became smaller and the
color became lighter, and finally showed the phenotype of male sterility in comparison to the wild type. I<sub>2</sub>-KI
staining showed that the pollen was malformed and only a little was active. Scanning electron microscopy observation showed that the exine waxy layer of the <i>ms10</i> anther decreased, suggesting that the protective effect on
pollen was decreased. This may be one of the reasons leading to the phenotype of male sterility. Finally, the pollen
showed shrinkage and collapsed, and the structure of germinating pore cover disappeared. This may be the result
of sterility. Genetic analysis showed that the male sterility phenotype of the mutant was controlled by a single
recessive nuclear gene. <i>MS10</i> was mapped between the molecular markers IND37 and IND51 on chromosome 4,
with a physical distance of 178.6 kb. These results lay the foundation for further studies on <i>MS10</i>.
KW  - Gene mapping; male sterility; rice (<i>Oryza sativa</i> L.)

DO  - 10.32604/phyton.2021.014505