@Article{phyton.2021.012862,
AUTHOR = {Lucía Isabel Chávez-Ortiz, José Francisco Morales-Domínguez, Araceli Rodríguez-Sahagún, Eugenio Pérez-Molphe-Balch},
TITLE = {<i>In Vitro</i> Propagation of <i>Agave guiengola</i> Gentry Using Semisolid Medium and Temporary Immersion Bioreactors},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {3},
PAGES = {1003--1013},
URL = {http://www.techscience.com/phyton/v90n3/41926},
ISSN = {1851-5657},
ABSTRACT = {<i>Agave guiengola</i>Gentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status
is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes,
but it could have other uses if more plants were available. <i>In vitro</i> propagation by enhanced axillary sprouting
from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l<sup>–1</sup> BA, obtaining a mean
of 3.7 shoots per explant. Other interesting responses were observed, such as nodular callus induction using combinations of BA and 2,4-Dichlorophenoxyacetic acid (2,4-D); root induction without Plant Growth Regulators
(PGR); and generation of shoot clusters. These clusters constituted an excellent explant for micropropagation
in temporary immersion bioreactors, obtaining a propagation rate of 43 shoots per explant with 1 min immersion
and 6 h immersion frequencies. All new plants rooted and survived the transfer to soil. This study developed an in
vitro propagation scheme to produce individuals that can be used either for reforestation, economical purposes, or
to carry out studies in this species to assess its full potential, avoiding exploitation from wild plants.},
DOI = {10.32604/phyton.2021.012862}
}