@Article{phyton.2021.014437,
AUTHOR = {Qi Zhang, Pengtao Li, Aiying Liu, Shaoqi Li, Quanwei Lu, Qun Ge, Junwen Li, Wankui Gong, Xiaoying Deng, Haihong Shang, Yuzhen Shi, Youlu Yuan},
TITLE = {Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from <i>Gossypium hirsutum</i> × <i>Gossypium barbadense</i> via Bulked Segregant RNA-Sequencing},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {3},
PAGES = {837--858},
URL = {http://www.techscience.com/phyton/v90n3/41930},
ISSN = {1851-5657},
ABSTRACT = {Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for
fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC<sub>5</sub>F<sub>3:5</sub> CSSLs (chromosome segment substitution lines) from <i>Gossypium hirsutum</i> CCRI36 × <i>G. barbadense</i>
Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC<sub>7</sub>F<sub>2</sub> with its recurrent
parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed
and sequenced, including two parents (CCRI36 and MBI9915) and two extreme pools at 20 DPA (days post anathesis). Through multiple comparisons, 536 DEGs (differentially expressed genes) were overlapped at 20 DPA.
Allelic-polymorphism comparison in mRNA sequences revealed 831 highly probable SNPs between two extreme
pools related to fiber strength. Linkage analysis was performed between two extreme pools with SNP-index method.
Eighteen correlated regions with 1981 annotation genes were obtained between two pools at 20 DPA, of which
12 common DEGs were similarly identified both between two parents and two pools. One gene (<i>Gh_A07G0837</i>)
in the candidate region related to fiber strength was differentially expressed in both parents and extreme pools
and involved in fiber strength development through reactive oxygen species (ROS) activity. Co-expression analysis
of <i>Gh_A07G0837</i> showed that <i>Gh_A07G0837</i> may cooperate with other genes to regulate fiber strength. The reliability
of BSR-seq results was validated by the quantitative real-time PCR (qRT-PCR) experiments on 5 common DGEs
20 DPA. Co-expressed analysis results indicated that there were some genes expressed especially low in MBI9915,
resulting in good fiber strength. Focusing on bulked segregant analysis on the extreme pools derived from superior
CSSL population, this study indicates that BSR-seq can be efficiently applied on rapid identification of candidate genes
related to fiber strength, which make contributions to our understanding of fiber quality formation in cotton.},
DOI = {10.32604/phyton.2021.014437}
}