@Article{phyton.2021.013805,
AUTHOR = {Qian Sun, Toshihiko Yamada, Yulai Han, Tetsuo Takano},
TITLE = {Differential Responses of <i>NHX1</i> and <i>SOS1</i> Gene Expressions to Salinity in two <i>Miscanthus sinensis</i> Anderss. Accessions with Different Salt Tolerance},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {3},
PAGES = {827--836},
URL = {http://www.techscience.com/phyton/v90n3/41936},
ISSN = {1851-5657},
ABSTRACT = {The lignocellulosic crop <i>Miscanthus</i> spp. has been identified as a good candidate for biomass production. The
responses of <i>Miscanthus sinensis</i> Anderss. to salinity were studied to satisfy the needs for high yields in marginal
areas and to avoid competition with food production. The results indicated that the relative advantages of the
tolerant accession over the sensitive one under saline conditions were associated with restricted Na<sup>+</sup> accumulation
in shoots. Seedlings of two accessions (salt-tolerant ‘JM0119’ and salt-sensitive ‘JM0099’) were subjected to 0
(control), 100, 200, and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes
involved in Na<sup>+</sup> accumulation in <i>M. sinensis</i>. The adaptation responses of genes encoding for Na<sup>+</sup>
/H<sup>+</sup> antiporters,
<i>NHX1</i> and <i>SOS1</i> to NaCl stress were examined in JM0119 and JM0099.The cDNA sequences of genes examined
were highly conserved among the relatives of <i>M. sinensis</i> based on the sequencing on approximate 600 bp-long
cDNA fragments obtained from degenerate PCR. These salt-induced variations of gene expression investigated by
quantitative real-time PCR provided evidences for insights of the molecular mechanisms of salt tolerance in
<i>M. sinensis</i>. The expression of <i>NHX1</i> was up-regulated by salt stress in JM0119 shoot and root tissues. However,
it was hardly affected in JM0099 shoot tissue except for a significant increase at the 100 mM salt treatment, and it
was salt-suppressed in the JM0099 root tissue. In the root tissue, the expression of <i>SOS1</i> was induced by the high
salt treatment in JM0119 but repressed by all salt treatments in JM0099. Thus, the remarkably higher expression
of <i>NHX1</i> and <i>SOS1</i> were associated with the resistance to Na<sup>+</sup> toxicity by regulation of the Na<sup>+</sup> influx, efflux, and
sequestration under different salt conditions.},
DOI = {10.32604/phyton.2021.013805}
}