@Article{phyton.2021.015601,
AUTHOR = {Min Yan, Han Wang, Shaohua Liu, Ye Xu, Zizhuo Nie, Yifeng Zhou, Li Li},
TITLE = {Cloning and Bioinformatics Analysis of the <i>GlROP6</i> gene in <i>Glehnia littoralis</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {4},
PAGES = {1293--1300},
URL = {http://www.techscience.com/phyton/v90n4/42399},
ISSN = {1851-5657},
ABSTRACT = {Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length <i>GlROP6</i> gene was cloned based on <i>G. littoralis</i> transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the <i>GlROP6</i> gene were systematically analysed. Our results showed that the full-length <i>GlROP6</i> gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved, and included five G-box motifs (G1–G5), an effector binding region, a Rho insert region and a C-terminal hypervariable region. According to our phylogenetic analysis, the GlROP6 protein was closely related to the ROP protein of <i>Daucus carota</i> subsp. <i>Sativus</i>. Our quantitative real-time PCR results revealed that <i>GlROP6</i> was highly expressed in flower, and <i>GlROP6</i> expression was significantly upregulated in <i>G. littoralis</i> roots treated with NaCl. This study will facilitate investigations into the function of <i>GlROP</i> genes in response to salt stress in <i>G. littoralis</i>.},
DOI = {10.32604/phyton.2021.015601}
}