TY  - EJOU
AU  - Wei, Wenjie 
AU  - Hu, Yulan 
AU  - Yang, Wenjuan 
AU  - Li, Xiaoli 
AU  - Wei, Jiali 
AU  - Hu, Xiangyang 
AU  - Li, Ping 

TI  - S-Nitrosoglutathion Reductase Activity Modulates the Thermotolerance of Seeds Germination by Controlling ABI5 Stability under High Temperature
T2  - Phyton-International Journal of Experimental Botany

PY  - 2021
VL  - 90
IS  - 4
SN  - 1851-5657

AB  - Seed germination or dormancy status is strictly controlled by endogenous phytohormone and exogenous environment signals. Abscisic acid (ABA) is the important phytohormone to suppress seed germination. Ambient high temperature (HT) also suppressed seed germination, or called as secondary seed dormancy, through upregulating ABI5, the essential component of ABA signal pathway. Previous result shows that appropriate nitric oxide (NO) breaks seed dormancy through triggering S-nitrosoglutathion reductase (GSNOR1)-dependent S-nitrosylation modification of ABI5 protein, subsequently inducing the degradation of ABI5. Here we found that HT induced the degradation of GSNOR1 protein and reduced its activity, thus accumulated more reactive nitrogen species (RNS) to damage seeds viability. Furthermore, HT increased the S-nitrosylation modification of GSNOR1 protein, and triggered the degradation of GSNOR1, therefore stabilizing ABI5 to suppress seed germination. Consistently, the ABI5 protein abundance was lower in the transgenic line overexpressing <i>GSNOR1</i>, but higher in the <i>gsnor</i> mutant after HT stress. Genetic analysis showed that GSNOR1 affected seeds germination through ABI5 under HT. Taken together, our data reveals a new mechanism by which HT triggers the degradation of GSNOR1, and thus stabilizing ABI5 to suppress seed germination, such mechanism provides the possibility to enhance seed germination tolerance to HT through genetic modification of GNSOR1.
KW  - Seed germination; ambient high temperature; GSNOR1; S-nitrosoglutathion; ABI5

DO  - 10.32604/phyton.2021.016134