TY  - EJOU
AU  - Zhang, Hecui 
AU  - Lian, Xiaoping 
AU  - Zhang, Yizhong 
AU  - Zuo, Tonghong 
AU  - Yuan, Chongmo 
AU  - Xie, Qinqin 
AU  - Zhu, Liquan 

TI  - Expression and Interaction Analysis of <i>FAZ1 Protein</i> in <i>Brassica oleracea</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2021
VL  - 90
IS  - 6
SN  - 1851-5657

AB  - To identify and characterize genes involved in reproductive tissue abscission in <i>Brassica oleracea</i>, the transcript data of pollinated pistil was analyzed. A differentially expressed gene, named <i>BoFAZ1(FLOWER ABSCISSION ZONE1)</i> was identified, which contains one exon and encompass a 139aa. Furthermore, a T-DNA insertion mutant (SALK_302_G01) (<i>faz1</i> mutant) was obtained from <i>Arabidopsis thaliana</i> mutant library. Floral organ shedding from mutants was delayed and a V-shaped structure in the boundary region between the stalk and torus of the sepal abscission zone was obtained in f<i>az1</i> mutant. The cell density of this structure was lower than that of the corresponding region in the wild-type control. In the transgenic plants, the normal development of the stalk zone of <i>faz1</i> was recovered completely by transforming a 1919-bp DNA fragment of <i>BoFAZ1</i> into the <i>faz1</i> mutant. In Addition, our data showed that <i>BoFAZ1</i> was expressed in mature pollen grains, but not in the bracts, roots, stems, leaves, and sepals. Its expression in the filaments, stigma, and pistil exfoliation layer gradually increased after pollination. Subcellular localization experiments showed that BoFAZ1 was located in the cell membrane. A myristoylation site was found at the N-terminus of <i>BoFAZ1</i>. Removal of this site resulted in protein dislocation in the cytoplasm, cell membrane and nucleus. Finally, a yeast two-hybrid test indicated that BoH3.2 (histone H3.2), a protein involved in abscission zone development, interacted with BoFAZ1. This interaction was verified by a GST pull-down assay. In summary, our data indicated that <i>BoFAZ1</i> was involved in the formation of the pistil abscission zone in <i>B. oleracea</i>.
KW  - Abscission; AZ; <i>B. oleracea</i>; flower development organ boundary

DO  - 10.32604/phyton.2021.015852