@Article{phyton.2021.016004,
AUTHOR = {Eliana Arias-Pérez, Carlos Alberto Lecona-Guzmán, Federico Antonio Gutiérrez-Miceli, Joaquín Adolfo Montes-Molina, Nancy Ruiz-Lau},
TITLE = {Encapsulation of Immature Somatic Embryos of <i>Coffea arabica</i> L. for <i>in Vitro</i> Preservation},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {90},
YEAR = {2021},
NUMBER = {6},
PAGES = {1741--1748},
URL = {http://www.techscience.com/phyton/v90n6/43028},
ISSN = {1851-5657},
ABSTRACT = {The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (<i>Coffea arabica</i> L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L<sup>−1</sup> of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L<sup>−1</sup> of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L<sup>−1</sup> BA + 1 mg · L<sup>−1</sup> NAA + 3% (w/v) alginate, and MS + 7 mg · L<sup>−1</sup> BA + 5.7 mg · L<sup>−1</sup> indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl<sub>2</sub>). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments.},
DOI = {10.32604/phyton.2021.016004}
}