@Article{phyton.2022.021199,
AUTHOR = {Xiaofang Zeng, Guangzheng Li, Nu’an Liu, Yan Li, Jianrong Li, Xiaozhen Huang, Degang Zhao},
TITLE = {Identification and Characterization of a Novel Yellow Leaf Mutant <i>yl1</i> in Rice},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {11},
PAGES = {2419--2437},
URL = {http://www.techscience.com/phyton/v91n11/48784},
ISSN = {1851-5657},
ABSTRACT = {Leaf-color mutants play an important role in the study of chlorophyll metabolism, chloroplast development, and
photosynthesis system. In this study, the <i>yellow leaf 1</i> (<i>yl1</i>) rice mutant was identified from the ethyl methane
sulfonate-treated mutant progeny of Lailong, a glutinous <i>japonica</i> rice landrace cultivated in Guizhou Province,
China. Results showed that <i>yl1</i> exhibited yellow leaves with decreased chlorophyll content throughout the growth
period. Chloroplast development in the <i>yl1</i> mutant was disrupted, and the grana lamellae was loosely packed and
disordered. RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) analysis revealed
that the chlorophyll synthesis-related genes <i>OsCHLH</i>, <i>OsCHLM</i>, <i>OsCHLG</i>, <i>PORB</i>, and <i>YGL8</i>, as well as the chloroplast development-related genes <i>FtsZ</i>, <i>OsRpoTp</i>, and <i>RbcL</i>, were down-regulated in the <i>yl1</i> mutant. Genetic analysis revealed that the yellow leaf phenotype of <i>yl1</i> was controlled by recessive nuclear gene. By employing the
MutMap method, the mutation responsible for the phenotype was mapped to a 6.17 Mb region between
17.34 and 23.51 Mb on chromosome 3. Two non-synonymous single-nucleotide polymorphisms (SNPs) located
in the gene locus <i>LOC_Os03g31210</i> and <i>LOC_Os03g36760</i> were detected in this region. The two SNPs were
further confirmed by PCR and Sanger sequencing. The expression patterns of the two candidate genes indicated
that <i>LOC_Os03g36760</i> showed greater potential for functional verification. Subcellular protein localization
revealed that the encoded product of <i>LOC_Os03g36760</i> was localized in the nucleus, cytoplasm, and plasma membrane. These results will be useful for further characterization and cloning of the <i>yl1</i> gene, and for research on the
molecular mechanisms controlling biogenesis and chloroplast biochemical processes.},
DOI = {10.32604/phyton.2022.021199}
}