@Article{phyton.2022.021889,
AUTHOR = {Qiang Ai, Cuijing Liu, Mei Han, Limin Yang},
TITLE = {Selection and Verification of Reference Genes for qRT-PCR Analysis in <i>Iris domestica</i> under Drought},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {11},
PAGES = {2537--2548},
URL = {http://www.techscience.com/phyton/v91n11/48795},
ISSN = {1851-5657},
ABSTRACT = {<i>Iris domestica</i> is a plant of the <i>Iridaceae</i> family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of <i>I. domestica</i> by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of <i>I. domestica</i> to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in <i>I. domestica</i> under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that <i>EF1β</i> was the most stable reference genes under drought stress and in different tissues. To validate further the stability of the identified reference genes, the expression patterns of <i>VP</i> gene in <i>I. domestica</i> was analysed. These results will be conducive to more accurate quantification of gene expression levels in <i>I. domestica</i>.},
DOI = {10.32604/phyton.2022.021889}
}