TY  - EJOU
AU  - Ai, Qiang 
AU  - Liu, Cuijing 
AU  - Han, Mei 
AU  - Yang, Limin 

TI  - Selection and Verification of Reference Genes for qRT-PCR Analysis in <i>Iris domestica</i> under Drought
T2  - Phyton-International Journal of Experimental Botany

PY  - 2022
VL  - 91
IS  - 11
SN  - 1851-5657

AB  - <i>Iris domestica</i> is a plant of the <i>Iridaceae</i> family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of <i>I. domestica</i> by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of <i>I. domestica</i> to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in <i>I. domestica</i> under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that <i>EF1β</i> was the most stable reference genes under drought stress and in different tissues. To validate further the stability of the identified reference genes, the expression patterns of <i>VP</i> gene in <i>I. domestica</i> was analysed. These results will be conducive to more accurate quantification of gene expression levels in <i>I. domestica</i>.
KW  - <i>Iris domestica</i>; gene expression; qRT-PCR; reference genes; drought

DO  - 10.32604/phyton.2022.021889