TY - EJOU
AU - Zhang, Ting
AU - Li, Ping
AU - Wei, Jiali
TI - Transcriptome Analysis via RNA Sequencing Reveals the Molecular Mechanisms Underlying the Hedera helix Response to High Temperature
T2 - Phyton-International Journal of Experimental Botany
PY - 2022
VL - 91
IS - 11
SN - 1851-5657
AB - Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress. Two Hedera helix cultivars, heat-tolerant (HT) ‘Jessica’ and heat-sensitive (HS) ‘Shamrock’, were used for differences analyses of transcriptome. We detected 6179 differentially expressed genes (DEGs) and 5992 DEGs in ‘Jessica’ and ‘Shamrock’ to heat stress, respectively. Among these, 1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties, resulting in enhancement of various pathways such as biosynthesis of secondary metabolites, glyoxylate dicarboxylate metabolism, and protein processing in endoplasmic reticulum (PPER), RNA transport, respectively. Among the common downregulated DEGs, 72 TFs in 25 gene families were found, including members of the MYB and MYB-related families, the bHLH family, etc. In ‘Jessica’ (HT), 634 unique up-regulated DEGs were identified, including genes associated with phenylpropanoid biosynthesis, starch and sucrose metabolism, biosynthesis of amino acids. Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature. Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR. This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance, which provided theoretical references for improving the heat tolerance of ivy.
KW - Hedera helix; RNA-sequencing; heat stress; heat tolerance; transcriptome
DO - 10.32604/phyton.2022.022421