@Article{phyton.2022.021631,
AUTHOR = {Minghui Cui, Limei Lin, Jie Zhang, Xin Song, Shuo Wang, Jing Dong, Xuelei Zhao, Yuehong Long, Zhaobin Xing},
TITLE = {The Effect of Methylation Modification of <i>MDD</i> on the Expression of Key Genes in the Process of Saponin Synthesis in <i>Eleutherococcus senticosus</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {12},
PAGES = {2649--2668},
URL = {http://www.techscience.com/phyton/v91n12/49483},
ISSN = {1851-5657},
ABSTRACT = {Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in <i>Eleutherococcus senticosus</i>. The results of bisulfite sequencing showed that there were three kinds of samples with a low (0.68%), medium (0.72%) and high (0.79%) DNA methylation ratio in the promoter of <i>MDD</i> in <i>E</i>. <i>senticosus</i>, respectively. The transcriptome sequencing results showed that the expression of <i>MDD</i> in <i>E</i>. <i>senticosus</i> was significantly up-regulated in the types with low DNA methylation ratios of <i>MDD</i> (<i>P</i> < 0.05). There was a significant negative correlation between the saponin content in <i>E</i>. <i>senticosus</i> and the DNA methylation ratio of <i>MDD</i> promoter (<i>P</i> < 0.05). The screening results of differentially expressed transcription factors among the three groups with different DNA methylation ratio showed that a total of 4 transcription factors could bind to 6 CpG sites. Protein-protein interaction analysis showed that <i>E</i>. <i>senticosus MDD</i> could interacted with other key enzymes in the process of terpenoid synthesis. In addition, it was found that the DNA methylation of <i>MDD</i> promoter was mainly regulated by DNA methyltransferase. These results demonstrated that under the action of DNA methyltransferase, the changes of DNA methylation of <i>MDD</i> promoter could regulate its own expression level by affecting the combination ability of transcription factors, and then affected the expression of other genes in terpenoid synthesis pathway. The synthesis and accumulation of saponins in <i>E</i>. <i>senticosus</i> was also changed.},
DOI = {10.32604/phyton.2022.021631}
}