@Article{phyton.2022.021474,
AUTHOR = {Huijun Jiang, Kaien Zhai, Xiaofan Ye, Tianwei Hu, Jieming Jiang, Xiaoqiu Dong, Weihuai Pan, Jianwei Pan, Jianxin Shou},
TITLE = {The Endosperm-Specific Expression of <i>YUCCA</i> Genes Enhances Rice Grain Filling},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {12},
PAGES = {2633--2648},
URL = {http://www.techscience.com/phyton/v91n12/49495},
ISSN = {1851-5657},
ABSTRACT = {Grain filling is a crucial process that affects yield in rice (<i>Oryza sativa</i> L.). Auxin biosynthesis and signaling are
closely related to rice yield; therefore, it is important to understand the effects of auxin biosynthesis on rice grain
filling to improve crop yield. In this study, we used physiological and molecular strategies to identify the roles of
auxin in rice grain filling. Exogenous application of auxin (IAA) or auxin analogues (2, 4-D) to young spikelets
and flag leaves improved the seed-setting rate and yield per spike. Furthermore, real-time quantitative PCR assays
confirmed that nine members of the <i>OsYUCCA</i> family of auxin biosynthetic genes were upregulated during grain
filling, implication that auxin biosynthesis plays a major role in grain development. The specific expression of
either Arabidopsis <i>AtYUCCA1</i> or <i>OsYUCCA2</i> in the endosperm or leaves resulted in increased expression of
<i>OsIAA</i> genes and auxin content of seeds, as well as increased grain filling and seed-setting rate. This result establishes that the auxin content in grains and leaves is important for grain development. Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.},
DOI = {10.32604/phyton.2022.021474}
}