TY  - EJOU
AU  - Jing, Danlong 
AU  - Wang, Nan 
AU  - Lu, Nan 
AU  - Qu, Guanzheng 
AU  - Liang, Guolu 
AU  - Ma, Wenjun 
AU  - Zhang, Shougong 
AU  - Wang, Junhui 

TI  - Global Lysine Acetylome Analysis of Flower Bud Development in <i>Catalpa bungei</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2022
VL  - 91
IS  - 3
SN  - 1851-5657

AB  - Lysine acetylation, a reversible and dynamic post-translational modification, plays pivotal roles in regulating many biological processes in plants. However, the information of lysine acetylation during flower development of woody angiosperm remains unclear. Herein, we identified a total of 667 lysine acetylation sites in 467 proteins in flower buds of <i>Catalpa Bungei</i>. The acetylated proteins were mainly involved in the pathways of carbon metabolism, glycometabolism and oxidative phosphorylation. Using functional enrichment analysis, 61 and 13 acetylated proteins were involved in the glycometabolism and oxidative phosphorylation pathways, respectively. This suggests that lysine acetylated proteins play critical roles in the energy metabolism pathways. Accordingly, the protein–protein interactions were also highly enriched in the carbon metabolic pathway. Integrative analysis of lysine acetylome and transcriptome showed that transcript level changes of seven key lysine-acetylated proteins/genes involved in energy metabolism were significant different during floral transition. Importantly, compared with the normal flowering variety, the expression levels of phosphoribulokinase, phosphoenolpyruvate carboxykinase, ATP synthase and pyruvate dehydrogenase complex genes were significantly up-regulated at the stages of vegetative and transition buds during early flowering of <i>C. bungei</i>. These data represent the first lysine acetylome in the <i>C. bungei</i> flowers, and can serve as an essential basis for exploring the function of lysine acetylated proteins in the flower bud development of plants.
KW  - Lysine acetylome; <i>Catalpa bungei</i>; flower development; energy metabolism

DO  - 10.32604/phyton.2022.017644