@Article{phyton.2022.018223,
AUTHOR = {Muslum S. Inal, Dilek Unal, Bengu Turkyilmaz Unal, Munir Ozturk},
TITLE = {Effect of Putrescine on Low-Temperature Acclimation in <i>Chlamydomonas reinhardtii</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {3},
PAGES = {583--598},
URL = {http://www.techscience.com/phyton/v91n3/45314},
ISSN = {1851-5657},
ABSTRACT = {Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae <i>Chlamydomonas reinhardtii</i>. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (<i>odc2</i>, and <i>spd1)</i>, heat shock proteins (hsp<i>70c, hsp90a</i>, and <i>hsp90c</i>), and PSII repair mechanisms (<i>psba, rep27</i>, and <i>tba1)</i> were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis showed that polyamine metabolism induced gene expression at low-temperatures in the first 24 h. In the same way, the gene expression of heat shock proteins (<i>hsp70c</i>, <i>hsp90a</i>, and <i>hsp90c</i>) decreased under low-temperature treatment for 72 h; however, application of putrescine enhanced the gene expression in the first 24 h. The results obtained indicated that molecular response in the first 24 h could be important for cold acclimation. The <i>psba</i> and <i>tba1</i> expressions were reduced under low-temperatures depending on the exposure time. In contrast, the exogenous putrescine enhanced the expression level of the <i>psba</i> response to low-temperature at 24 and 72 h. The results obtained in this study indicate that putrescine could play a role in the PS II repair mechanisms under low-temperature stress.},
DOI = {10.32604/phyton.2022.018223}
}