@Article{phyton.2022.018767,
AUTHOR = {Yuting Luan, Cong Xu, Xiaoxiao Wang, Daqiu Zhao, Jun Tao},
TITLE = {Selection of Stable Reference Genes for Quantitative Real-Time PCR on <i>Paeonia ostii</i> T. Hong et J. X. Zhang Leaves Exposed to Different Drought Stress Conditions},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {5},
PAGES = {1045--1059},
URL = {http://www.techscience.com/phyton/v91n5/46606},
ISSN = {1851-5657},
ABSTRACT = {The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, <i>ACT1</i>, <i>ACT2</i>, <i>GAPDH</i>, <i>eIF1</i>, <i>eIF2</i>, <i>α-TUB</i>, <i>β-TUB</i>, <i>TBP</i>, <i>RNA Pol II</i> and <i>RP II</i>, were calculated for a suitable reference gene selection in <i>Paeonia ostii</i> T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed that <i>RNA Pol II</i> was the most stable gene and <i>eIF2</i> was the least stable one. In addition, the geNorm program provided the optimal choice of two reference gene combination, <i>RNA Pol II</i> and <i>β-TUB</i>, for qRT-PCR normalization in <i>P. ostii</i> subjected to different drought stress levels. Our research provided convenience for gene expression analysis in <i>P. ostii</i> under drought stress and promoted research of effective methods to alleviate <i>P. ostii</i> drought stress in the future.},
DOI = {10.32604/phyton.2022.018767}
}