@Article{phyton.2022.019219,
AUTHOR = {Lifen Zheng, Fangfang Chen, Yuanjun Li, Changfu Li},
TITLE = {Identification of a Unique Germacrene A Oxidase from <i>Xanthium strumarium</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {5},
PAGES = {989--998},
URL = {http://www.techscience.com/phyton/v91n5/46610},
ISSN = {1851-5657},
ABSTRACT = {8,12-sesquiterpene lactones (STLs) are an important class of natural products with unique pharmaceutical activities. For years the pathway leading to 8,12-STLs remains an enigma. <i>Xanthium strumarium</i> accumulates abundant 8,12-STLs, and xanthatin is a characteristic 8,12-STL in it. Xanthatin has been previously postulated to be derived from germacrene A, but the steps from germacrene A to xanthatin are unknown. As part of an effort to understand the xanthatin biosynthetic pathway. This study reports the characterization of a unique germacrene A oxidase (XsGAO) from <i>X. strumarium</i>. Unlike a classical GAO enzyme, which is known to catalyze a three-step oxidation of germarene A to yield germacrene A acid (GAA), surprisingly, XsGAO catalyzed only one-step conversion of germacrene A to germacrene A alcohol. We discussed that GAO may be pressured to acquire a novel activity during the evolutionary path for the biosynthesis of 8,12-STLs.},
DOI = {10.32604/phyton.2022.019219}
}