@Article{phyton.2022.018853,
AUTHOR = {Yuzhe Zhang, Guanglong Li, Shaowang Hu, Jinfeng Liu, Yushi Jiang, Siyan Liu, Shuyan Guan, Jing Qu, Dan Yao, Andi Shi, Yixuan Liu},
TITLE = {Cloning and Drought Resistance Analysis of Soybean <i>GmHsps_p23-like</i> Gene},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {6},
PAGES = {1183--1198},
URL = {http://www.techscience.com/phyton/v91n6/46796},
ISSN = {1851-5657},
ABSTRACT = {Soybean (<i>Glycine max</i> (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean <i>GmHsps_p23-like</i> gene was successfully cloned by RT-PCR, the protein encoded by the <i>GmHsps_p23-like</i> gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. <i>Agrobacterium</i>-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance physiological and biochemical index detection were performed on the T<sub>2</sub> generation positive transgenic soybean plants identified by PCR and Southern hybridization. The results showed that the overexpression vector plant <i>GmHsps_p23-like</i> gene expression increased. After rehydration, the transgenic overexpression plants returned to normal growth, and the damage to the plants was low. After drought stress, the SOD and POD activities and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. The drought resistance of the overexpressed soybeans under drought stress was higher than that of the control group, and had a stronger drought resistance. It showed that the expression of soybean <i>GmHsps_p23-like</i> gene can improve the drought resistance of soybean. The cloning and functional verification of soybean <i>GmHsps_p23-like</i> gene had not been reported yet. This is the first time that PCR technology has been used to amplify the soybean <i>GmHsps_p23-like</i> gene and construct an expression vector for this gene. This research has laid the foundation for transgenic technology to improve plant drought resistance and cultivate new drought-resistant transgenic soybean varieties.},
DOI = {10.32604/phyton.2022.018853}
}