@Article{phyton.2022.020010,
AUTHOR = {Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang},
TITLE = {Bioinformatics Analysis of Disease Resistance Gene <i>PR1</i> and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {7},
PAGES = {1445--1464},
URL = {http://www.techscience.com/phyton/v91n7/47021},
ISSN = {1851-5657},
ABSTRACT = {In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-<i>PR1</i> containing the disease-resistant gene <i>PR1</i> was constructed by means of genetic engineering, and the <i>PR1</i> gene was genetically transformed to contain the <i>PR1</i> gene through the pollen tube method. In <i>CryAb-8Like</i> transgenic high-generation T<sub>7</sub> receptor soybean, a new material that is resistant to insects and diseases is obtained. For T<sub>2</sub> transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed that there were 9 positive plants in the routine PCR test of T<sub>2</sub> generation. In Southern Blot hybridization, both <i>PR1</i> and <i>CryAb-8Like</i> genes are integrated in soybeans in the form of single copies. Fluorescence quantitative PCR showed that the expression levels of <i>PR1</i> and <i>CryAb-8Like</i> genes are different in different tissues. The average expression levels of <i>PR1</i> gene in plant roots, stems, and leaves are 2.88, 1.54, and 5.26, respectively. <i>CryAb-8Like</i> genes are found in roots, stems, and leaves. The average expression levels were 1.36, 1.39, and 4.25, respectively. The insectivorous rate of the <i>CryAb-8Like</i> gene in outdoor plants with positive insect resistance identification was 3.78%. The disc partition method was used indoors for pest resistance identification, and the bud length of transformed plants increased significantly. The average mortality rate of untransformed plants in indoor disease resistance identification was as high as 56.66%, and the average mortality rate of plants transformed with <i>PR1</i> gene was 10.00%, and disease resistance was significantly improved. Therefore, a new material with resistance to diseases and insects is obtained.},
DOI = {10.32604/phyton.2022.020010}
}