@Article{phyton.2022.018854,
AUTHOR = {Jing Qu, Shuang Liu, Peng Jiao, Zhenzhong Jiang, Jianbo Fei, Shuyan Guan, Yiyong Ma},
TITLE = {Development of a New Cold-Tolerant Maize (<i>Zea mays</i> L.) Germplasm Using the <i>ICE1</i> Gene from <i>Arabidopsis thaliana</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {91},
YEAR = {2022},
NUMBER = {8},
PAGES = {1709--1719},
URL = {http://www.techscience.com/phyton/v91n8/47320},
ISSN = {1851-5657},
ABSTRACT = {To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (<i>ICE1</i>) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the <i>ICE1</i> gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using <i>Agrobacterium tumefaciens</i>-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the <i>ICE1</i> gene was integrated into the maize (<i>Zea mays</i> L.) genomes of the three T1 generations. Under low temperature-stress conditions (4°C), the relative conductivity levels decreased by 27.51%–31.44%, the proline concentrations increased by 12.50%–17.50%, the malondialdehyde concentrations decreased by 16.78%–18.37%, and the peroxidase activities increased by 19.60%–22.89% in the T1 lines compared with those of the control. A real-time quantitative PCR analysis showed that the <i>ICE1</i> gene was ectopically expressed in the roots, stems, and leaves of the T1 lines. <i>ICE1</i> positively regulates the expression of the <i>CBF</i> genes in response to cold stress. Thus, this study showed the successful transformation of maize with the <i>ICE1</i> gene, resulting in the generation of a new maize germplasm that had increased tolerance to cold stress.},
DOI = {10.32604/phyton.2022.018854}
}