TY  - EJOU
AU  - Ying, Songbei 
AU  - Jing, Sasa 
AU  - Cheng, Leheng 
AU  - Sun, Haiqing 
AU  - Tian, Yuan 
AU  - Zhi, Lulu 
AU  - Li, Ping 

TI  - Allantoin Alleviates Seed Germination Thermoinhibition in <i>Arabidopsis</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2022
VL  - 91
IS  - 9
SN  - 1851-5657

AB  - Allantoin as the metabolite of purine catabolism can store and remobilize nitrogen for plant growth and development. However, emerging evidence suggests it also contributes to plant tolerance to stress response through altering abscisic acid (ABA) and reducing reactive oxygen species (ROS) level. 1-CYS PEROXIREDOXIN (PER1) is a seed-specific antioxidant that enhances seed longevity through scavenging ROS over-accumulation. High temperature (HT) suppresses seed germination and induces seed secondary dormancy, called as seed germination thermoinhibition. However, the mechanism that allantoin and <i>PER1</i> regulate seed germination thermoinhibition remains unknown. In this study, we reported that allantoin treatment enhances seed germination under HT stress. Consistently, the <i>aln</i> mutants displayed higher seed germination, as well as more accumulation of endogenous allantoin, than that of wild-type control. Further biochemical and genetic analyses showed that allantoin reduces ABA content under HT, and allantoin targets <i>PER1</i> to efficiently scavenge HT-induced ROS accumulation, meanwhile, the function of allantoin requires <i>PER1</i> during seed gemination thermotolerance. Collectively, our finding proposes a novel function of allantoin in enhancing seed germination tolerance to HT, and uncovers the underlying mechanism by which allantoin regulates seed germination through altering ABA metabolism and <i>PER1</i>-mediated ROS level under HT stress.
KW  - Seed germination; high temperature; allantoin; <i>PRE1</i>

DO  - 10.32604/phyton.2022.022679