@Article{phyton.2022.022384,
AUTHOR = {Yanyan Zhao, Yanpeng Ding, Bailin Duan, Qingzhou Xie},
TITLE = {<i>GhSCL4</i> Acts as a Positive Regulator in Both Transgenic <i>Arabidopsis</i> and Cotton during Salt Stress},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {1},
PAGES = {1--15},
URL = {http://www.techscience.com/phyton/v92n1/49586},
ISSN = {1851-5657},
ABSTRACT = {GRAS transcription factors play important roles in plant abiotic stress response, but their characteristics and
functions in cotton have not been fully investigated. A cotton SCL4/7 subgroup gene in the GRAS family,
<i>GhSCL4</i>, was found to be induced by NaCl treatments. Nuclear localization and transactivation activity of <i>GhSCL4</i>
indicate its potential role in transcriptional regulation. Transgenic Arabidopsis thaliana over-expressing <i>GhSCL4</i>
showed enhanced resistance to salt and osmotic stress. What’s more, the transcript levels of salt stress-induced
genes (AtNHX1 and AtSOS1) and oxidation-related genes (AtAPX3 and AtCSD2) were more highly induced in
the <i>GhSCL4</i> over-expression lines than in wild type after salt treatment. Furthermore, silencing of <i>GhSCL4</i>
resulted in reduced salt tolerance in cotton caused by reactive oxygen species (ROS) enrichment under salt treatment, and antioxidant enzyme activities were accordingly significantly reduced in GhSLC4-silenced lines. These
results indicated that <i>GhSCL4</i> enhances salt tolerance of cotton by detoxifying ROS. In addition, the transient
expression assay confirmed an interactive relationship between <i>GhSCL4</i> and GhCaM7, which indicated that salt
tolerance conferred by <i>GhSCL4</i> might be associated with salt-induced Ca<sup>2+</sup>/CaM7-mediated signaling. Taken
together, <i>GhSCL4</i> acts as a positive regulator in cotton during salt stress that is potentially useful for engineering
salt-tolerant cotton.},
DOI = {10.32604/phyton.2022.022384}
}