@Article{phyton.2023.030396,
AUTHOR = {Laura Isabel Arias-Rodríguez, Martha Alicia Rodríguez-Mendiola, Carlos Arias-Castro, Federico Antonio Gutiérrez Miceli, Diana Reséndez Pérez, María Celina Luján Hidalgo, Juan José Villalobos Maldonado, Norma Alejandra Mancilla Margalli},
TITLE = {Comparison of Apigenin, Quercetin and Kaempferol Accumulation and Total Flavonoid Content in Leaves, Embryogenic Cultures and Cell Suspension Cultures of Parsley (<i>Petroselinum crispum</i>)},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {10},
PAGES = {2807--2823},
URL = {http://www.techscience.com/phyton/v92n10/54193},
ISSN = {1851-5657},
ABSTRACT = {In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue
engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of
bone regeneration. In our study, we specifically investigated the extract of <i>Petroselinum crispum</i>, a plant known
for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly embryogenic cultures, and
suspension cell cultures. The TFC, measured in mg quercetin equivalent per gram of dry weight (QE mg/g dw),
was found to be 53.81 ± 0.97 for 52 g dw of leaves (1 kg plant fw), 31.10 ± 1.52 for 420 g dw of embryogenic
cultures, and 11.80 ± 0.76 for 210 g dw of cell suspensions. Notably, only the leaves and embryogenic cultures
showed significant accumulation of osteogenic flavonoids, with apigenin levels of 1.8 ± 0.3 and 0.32 ±
0.02 mg/g dw, and kaempferol levels of 1.0 ± 0.18 and 0.2 ± 0.01 mg/g dw, respectively. Our findings indicate
that embryogenic cultures have the potential to produce up to 40% more apigenin in 12 weeks compared to
1 kg of parsley plant, thanks to their superior biomass reproductive competence. These results present a promising biotechnological approach for generating extracts enriched with bioactive compounds, which can be further
utilized in in vitro osteogenic testing.},
DOI = {10.32604/phyton.2023.030396}
}