@Article{phyton.2022.025467,
AUTHOR = {Lunzeng Huang, Hongsheng Gao, Ning Jiang, Yunhong Xu, Zijian Gong, Lele Chen, Shijie Xue, Xiaoyan Li, Ruichao Liu, Bei Li, Hongxia Zhang, Chunyan Yu, Xiaotong Guo},
TITLE = {Genome Wide Characterization of <i>CBL-CIPK</i> Family Genes and Their Responsive Expression in <i>Rosa chinensis</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {2},
PAGES = {349--368},
URL = {http://www.techscience.com/phyton/v92n2/50127},
ISSN = {1851-5657},
ABSTRACT = {Calcium (Ca<sup>2+</sup>) plays a pivotal role in various signal transduction pathways. Calcineurin B-like proteins (CBLs) are a unique group of Ca<sup>2+</sup> sensors that decode Ca<sup>2+</sup> signals by activating the plant specific protein kinase known as the CBL-interacting protein kinase (CIPK). In plants, the CBL-CIPK signaling network regulates multiple signals in response to different extracellular cues including abiotic stress. However, the genome wide annotation and expression patterns of CBLs and CIPKs in woody cutting flower plants are still unclear. In this study, a total number of 7 <i>CBL</i>s (<i>RcCBLs</i>) and 17 <i>CIPKs</i> (<i>RcCIPKs</i>) genes, divided into four and five subfamilies, respectively, were identified from the rose genome. All RcCBLs possess a classic elongation factor-hand (EF-hand) domain, while all RcCIPKs possess both the classic kinase and NAF domains. Most RcCBLs were predicted to be plasma membrane localized, whereas most RcCIPKs were predicted to be cytoplasmic localized. Synteny analysis showed that one <i>RcCBL</i> gene pair and five <i>RcCIPK</i> gene pairs have gone through whole genome duplication events. Promoter <i>cis</i>-element prediction assays indicated that <i>RcCBLs</i> and <i>RcCIPKs</i> could function in different abiotic stress responses in rose plants. Further quantitative real-time PCR analysis demonstrated that <i>RcCBLs</i> and <i>RcCIPKs</i> were expressed in different organs with overlapped but distinct patterns in response to various abiotic stresses. The findings in this work will provide fundamental information and gene resources for further functional research on <i>RcCBLs</i> and <i>RcCIPKs</i>.},
DOI = {10.32604/phyton.2022.025467}
}