TY  - EJOU
AU  - Jiang, Zhishu 
AU  - Gan, Cong 
AU  - Liu, Yulian 
AU  - Lin, Xiaoli 
AU  - Peng, Limei 
AU  - Song, Yongping 
AU  - Luo, Xiaowei 
AU  - Xu, Jie 

TI  - A Novel Dominant Allele from 93-11, <i>ES(4)</i>, Represses Reactive Oxygen Species Scavenging and Leads to Early-Senescence in Rice
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 3
SN  - 1851-5657

AB  - Senescence is the last developmental process in plant, which has an important impact on crop yield and quality. In this study, a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7 (W7). Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11. By linkage analysis, the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4, described as <i>ES(4)</i>. Through multiple backcrossing with W7, the near-isogenic line of <i>ES(4)</i> (NIL-ES(4)) was obtained. Compared with wild-type W7, NIL-ES(4) plants showed more sever senescence phenotype in both nature and dark conditions. In NIL plants, leaves turned yellow at the fully tillering stage; photosynthetic rate, pollen fertility and seed setting rate were decreased. Moreover, the malondialdehyde, proline content and relative conductivity in NIL-ES(4) were significantly higher than those in W7; both transcript level and activities of reactive oxygen species scavenging enzymes were repressed; H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup> were significantly accumulated. This study provides a basis for further cloning and function identification of <i>ES(4)</i>.
KW  - Rice; early-senescence; gene mapping; chlorophyll degradation; reactive oxygen species scavenging

DO  - 10.32604/phyton.2023.025266