TY  - EJOU
AU  - Du, Yeyao 
AU  - Song, Yang 
AU  - Zhang, Ye 
AU  - Fan, Sujie 
AU  - Zhang, Hanzhu 
AU  - Wang, Piwu 

TI  - The Improvement of Soybean Salt Tolerance by Overexpressed <i>GmPAO1</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 4
SN  - 1851-5657

AB  - Polyamines play an important regulatory role during plant growth and development and adversity stress, and polyamine oxidase (PAO) is involved in polyamine catabolism. In this study, an up-regulated polyamine oxidase gene <i>GmPAO1</i> was obtained by transcriptome sequencing analysis and screening at soybean seedling stages. Also, its expression pattern and function were analyzed. The identification results of transgenic <i>GmPAO1</i> soybean positive lines showed that the relative expression level of <i>GmPAO1</i> in the overexpressed lines was increased under salt stress. With increasing stress concentration, the seed germination rate decreased. However, the seed germination rate of the overexpressed lines was significantly higher than that of the control lines, and the phenotypic character of the root systems was also better than that of the control lines. The measurement of superoxide dismutase (SOD) and peroxidase (POD) activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities, significantly reducing the malondialdehyde content. Although the hydrogen peroxide content in the transformed plants gradually increased, the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing lines. Based on this, it was preliminarily judged that <i>GmPAO1</i> can improve soybean salt tolerance.
KW  - Soybean; polyamine oxidase; salt tolerance; overexpressed

DO  - 10.32604/phyton.2023.025503