@Article{phyton.2023.025918,
AUTHOR = {Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang},
TITLE = {Identification and Evaluation of Insect and Disease Resistance in Transgenic <i>Cry1Ab13-1</i> and <i>NPR1</i> Maize},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {4},
PAGES = {1257--1274},
URL = {http://www.techscience.com/phyton/v92n4/51256},
ISSN = {1851-5657},
ABSTRACT = {PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T<sub>2</sub>, T<sub>3</sub>, and T<sub>4</sub>) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene <i>Cry1Ab13-1</i> and disease resistance gene <i>NPR1</i>) and their corresponding wild type. Results indicated that the target genes <i>Cry1Ab13-1</i> and <i>NPR1</i> were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of <i>Cry1Ab13-1</i> and <i>NPR1</i> genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines exhibited resistance to large leaf spots and corn borer in the field evaluation compared to the wild type. Our study confirmed that <i>Cry1Ab13-1</i> and <i>NPR1</i> bivalent genes enhanced the resistance against maize borer and large leaf spot disease and can stably inherit. These findings could be exploited for improving other cultivated maize varieties.},
DOI = {10.32604/phyton.2023.025918}
}