TY  - EJOU
AU  - Xi, Yongjing 
AU  - Yang, Zhou 
AU  - Jin, Yukun 
AU  - Qu, Jing 
AU  - Guan, Shuyan 
AU  - Liu, Siyan 
AU  - Wang, Piwu 

TI  - Identification and Evaluation of Insect and Disease Resistance in Transgenic <i>Cry1Ab13-1</i> and <i>NPR1</i> Maize
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 4
SN  - 1851-5657

AB  - PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T<sub>2</sub>, T<sub>3</sub>, and T<sub>4</sub>) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene <i>Cry1Ab13-1</i> and disease resistance gene <i>NPR1</i>) and their corresponding wild type. Results indicated that the target genes <i>Cry1Ab13-1</i> and <i>NPR1</i> were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of <i>Cry1Ab13-1</i> and <i>NPR1</i> genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines exhibited resistance to large leaf spots and corn borer in the field evaluation compared to the wild type. Our study confirmed that <i>Cry1Ab13-1</i> and <i>NPR1</i> bivalent genes enhanced the resistance against maize borer and large leaf spot disease and can stably inherit. These findings could be exploited for improving other cultivated maize varieties.
KW  - Maize; <i>NPR1</i> gene; <i>Cry1Ab13-1</i> gene; disease resistance; insect resistance

DO  - 10.32604/phyton.2023.025918