@Article{phyton.2023.026227,
AUTHOR = {Pengfei Zhang, Yuqin Zhang, Qifeng Zhao, Tiequan Niu, Pengfei Wen, Jinjun Liang},
TITLE = {<i>VvAGAMOUS</i> Affect Development of Four Different Grape Species Ovary},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {4},
PAGES = {1125--1138},
URL = {http://www.techscience.com/phyton/v92n4/51257},
ISSN = {1851-5657},
ABSTRACT = {Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely ‘Musct Hambourg’ grape (<i>Vitis vinifera</i>), ‘Concord’ grape (<i>Vitis labrusca</i>), ‘ShanPuTao’ grape (<i>Vitis amurensis</i>) and ‘GongNiang2Hao’ grape (<i>Vitis amurensis</i> × <i>Vitis vinifera</i>) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene <i>VvAGAMOUS</i> (<i>VvAG</i>) and its development related genes <i>VvCRABS CLAW</i> (<i>VvCRC</i>) and <i>VvAGAMOUS-LIKE 11</i>  (<i>VvAGL11</i>) with similar functions during the development of different grape varieties were preliminarily explored using fluorescence quantitative test. The relationship between <i>VvAG</i> and <i>VvCRC</i>, <i>VvAG</i> and <i>VvAGL11</i> were analyzed using Y1H assay. Our results showed that there were obvious abdominal sutures on the surface of expect for ‘Musct Hambourg’ grape, and existing poly carpels. The ovary development of ‘ShanPuTao’ and ‘GongNiang2Hao’ grape was completed when the inflorescence length was less than 1 cm, while the ‘Concord’ and ‘Musct Hambourg’ grape were fully developed when the length of inflorescence was 3–4 and 4–5 cm, respectively. <i>VvAG</i> and <i>VvCRC</i> began to express in large quantities after the formation of stamen primordia, while <i>VvAGL11</i> during the forming of ovule primordia. Therefore, <i>VvAG</i> and <i>VvCRC</i> mainly regulated the development of stamens and carpels and also promote the development of ovules, while <i>VvAGL11</i> major regulated the development of ovules. The promoters of <i>VvCRC</i> and <i>VvAGL11</i> were bound by <i>VvAG</i>. This study provides an important theoretical basis for further research on the molecular mechanism of grape ovary development.},
DOI = {10.32604/phyton.2023.026227}
}