TY - EJOU
AU - An, Jia
AU - Wang, Xiang
AU - Jing, Yajiang
AU - Huang, Jianping
AU - Wang, Qilong
AU - Zhang, Gang
AU - Gao, Jing
AU - Peng, Liang
AU - Huang, Wenli
AU - Yan, Yonggang
TI - Subcellular Distribution and Chemical Forms of Cadmium in the Medicine Food Homology Plant Platycodon grandiflorum (Jacq.) A.DC.
T2 - Phyton-International Journal of Experimental Botany
PY - 2023
VL - 92
IS - 5
SN - 1851-5657
AB - Although Platycodon grandiflorum (Jacq.) A.DC. is a renowned medicine food homology plant, reports of excessive cadmium (Cd) levels are common, which affects its safety for clinical use and food consumption. To enable its Cd levels to be regulated or reduced, it is necessary to first elucidate the mechanism of Cd uptake and accumulation in the plant, in addition to its detoxification mechanisms. This present study used inductively couple plasma-mass-spectrometry to analyze the subcellular distribution and chemical forms of Cd in different tissues of P. grandiflorum. The experimental results showed that Cd was mainly accumulated in the roots [predominantly in the cell wall (50.96%–61.42%)], and it was found primarily in hypomobile and hypotoxic forms. The proportion of Cd in the soluble fraction increased after Cd exposure, and the proportion of insoluble phosphate Cd and oxalate Cd increased in roots and leaves, with a higher increase in oxalate Cd. Therefore, it is likely that root retention mechanisms, cell wall deposition, vacuole sequestration, and the formation of low mobility and low toxicity forms are tolerance strategies for Cd detoxification used by P. grandiflorum. The results of this study provide a theoretical grounding for the study of Cd accumulation and detoxification mechanisms in P. grandiflorum, and they can be used as a reference for developing Cd limits and standards for other medicine food homology plants.
KW - Cadmium; Platycodon grandiflorum; inductively coupled plasma-mass-spectrometry; subcellular distribution; chemical forms; heavy metal tolerance; medicine food homology
DO - 10.32604/phyton.2023.026525