@Article{phyton.2023.026830,
AUTHOR = {Jiali Wang, Xiangmei Chen, Xiaozhen Huang, Yichen Zhao, Degang Zhao},
TITLE = {Cloning and Function Identification of a Phytoene Desaturase Gene from <i>Eucommia ulmoides</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {5},
PAGES = {1377--1389},
URL = {http://www.techscience.com/phyton/v92n5/51865},
ISSN = {1851-5657},
ABSTRACT = {The phytoene desaturase (<i>PDS</i>) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting <i>PDS</i> expression leads to the appearance of mottled, chlorosis, or albino leaves. In this study, the CDS sequence of <i>EuPDS</i> (<i>Eucommia ulmoides</i> Phytoene Desaturase) was first cloned and then <i>PDS</i> was silenced in <i>Nicotiana benthamiana</i>. Result showed the expression level of <i>EuPDS</i> in leaves was higher than that in the roots and stems. In <i>N. benthamiana</i> leaves, which were treated by <i>Agrobacterium</i> for 24 h, photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of <i>PDS</i> was down-regulated during the period of emersion. This suggested that <i>EuPDS</i> could silence <i>PDS</i> of <i>N. benthamiana</i>, so as to cause the phenotype of leaf whitening. <i>PDS</i> is the main reporter gene involved in virus-induced gene silencing (VIGS). This study offered molecular evidence for identifying <i>PDS</i> gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in <i>E. ulmides</i>. It also laid a useful foundation for study on leaf discoloration mechanism of other woody plants.},
DOI = {10.32604/phyton.2023.026830}
}