@Article{phyton.2023.027133,
AUTHOR = {Peiling Wang, Xiaohong Xu, Yong Li, Hecui Zhang, Xuejie Zhang, Siru Zhou, Yimei Liu, Yunyan Feng, Tonghong Zuo, Liquan Zhu},
TITLE = {Genome-Wide Identification and Expression Analysis of the Phytocyanin Gene Family in <i>Nicotiana tabacum</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {5},
PAGES = {1469--1492},
URL = {http://www.techscience.com/phyton/v92n5/51869},
ISSN = {1851-5657},
ABSTRACT = {Phytocyanin (PC) is a class of plant-specific blue copper proteins involved in electron transport, plant growth, development, and stress resistance. However, PC proteins have not been systematically evaluated in tobacco plants. We determined the whole-genome sequences of the <i>PC</i> family in the tobacco cultivar ‘K326.’ The transcriptome data were used to analyze the expression of the <i>NtPC</i> family at different development stages and tissue-specific genes. Real-time fluorescence quantitative analysis was used to analyze the expression of the NtPC gene family under low temperature and methyl jasmonate stress. The tobacco <i>NtPC</i> family contained 110 members and was divided into four subfamilies: early nodulin-like protein (NtENODL), uclacyanin-like protein, stellacyanin1-like protein, and plantacyanin-like protein. According to phylogenetic and structural analyses, the <i>NtPC</i> family could be divided into eight structural types. Fifty-three NtPCs were randomly distributed on 22 of 24 tobacco chromosomes. Collinearity analysis revealed 33 pairs of genes belonging to the <i>NtPC</i> family. Gene ontology analysis showed that the PC genes are components of the plasma membrane and may participate in plasma membrane-related functions. The <i>NtPC</i> family contained numerous elements related to hormonal and abiotic stress responses and was specifically expressed in the tobacco prosperous, maturation, and budding periods. Tissue-specific expression analysis showed that some genes were tissue specific. The expression of <i>NtENODL58</i> and other genes was significantly induced by low-temperature and methyl jasmonate stress. Thus, the <i>NtPC</i> gene family plays an important role in plant stress response.},
DOI = {10.32604/phyton.2023.027133}
}