TY  - EJOU
AU  - Feng, Ziyang 
AU  - Cheng, Xinqi 
AU  - Wang, Tianwen 
AU  - Han, Yongchao 
AU  - Chen, Haihong 
AU  - Zhang, Xinyu 
AU  - Sun, Jie 
AU  - Zhang, Wei 
AU  - Liu, Feng 

TI  - Investigation of <i>GhFAT</i> Genes Related to Seed Oil Content and Fatty Acid Composition in <i>Gossypium hirsutum</i> L.
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 5
SN  - 1851-5657

AB  - Fatty Acyl-ACP thioesterase (FAT) is a key enzyme controlling oil biosynthesis in plant seeds. FATs can be divided into two subfamilies, FATA and FATB according to their amino acid sequences and substrate specificity. The Upland cotton genome contains 20 <i>GhFAT</i> genes, amongst which 6 genes were of the <i>GhFATA</i> subfamily and 14 of the <i>GhFATB</i> subfamily. The 20 <i>GhFAT</i> genes are unevenly distributed on 14 chromosomes. The <i>GhFATA</i> genes have 5 or 7 exons and the <i>GhFATB</i> genes have 6 or 7 exons. All <i>GhFAT</i> proteins have the conserved Acyl-ACP_TE domain and PLN02370 super family, the typical characteristics of plant thioesterases. Analyses of the expression level of <i>GhFATs</i> and the compositions of fatty acid in 5–60 days-post-anthesis seeds showed that the ratio of saturated fatty acids to unsaturated fatty acids was consistent with the expression profile of <i>GhFATB12</i>, <i>GhFATB3</i>, and <i>GhFATB10</i>; the ratio of monounsaturated fatty acid to polyunsaturated fatty acids was consistent with the expression profile of <i>GhFATA3</i>. The oil contents of mature cottonseeds were positively correlated with the contents of palmitic acid and linolenic acid as well as seed vigor. These results provide essential information for further exploring the role(s) of the specific <i>GhFATs</i> in determining oil biosynthesis and cottonseed compositions.
KW  - <i>Gossypium hirsutum</i>; fatty Acyl-ACP thioesterase; biological information; expression profile; seed vigor

DO  - 10.32604/phyton.2023.027542