@Article{phyton.2023.027870,
AUTHOR = {Xianrong Fu, Aiping Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen},
TITLE = {Brassinosteroid Biosynthetic Gene <i>CmDWF4</i> Regulates Bud Outgrowth in <i>Chrysanthemum morifolium</i>},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {6},
PAGES = {1681--1694},
URL = {http://www.techscience.com/phyton/v92n6/52304},
ISSN = {1851-5657},
ABSTRACT = {Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The <i>DWF4</i> gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identified a homologous gene of <i>DWF4</i> in chrysanthemum, <i>CmDWF4</i>. This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of <i>CmDWF4</i> in chrysanthemum was found to significantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosylase/hydrolase (XTH) family encoding genes associated with cell wall modification were up-regulated in <i>CmDWF4</i>-overexpressing lines. qRT-PCR assay confirmed the up-regulation of <i>CmXTH6</i>, <i>CmXTH23</i>, and <i>CmXTH28</i> in <i>CmDWF4</i>-overexpression line. Overall, this work establishes a mechanism by which BR biosynthetic gene <i>CmDWF4</i> promotes lateral bud outgrowth in chrysanthemum, possibly through regulating cell elongation and expansion.},
DOI = {10.32604/phyton.2023.027870}
}