TY  - EJOU
AU  - Hou, Cong 
AU  - Ning, Kang 
AU  - Wei, Xiuye 
AU  - Cheng, Yufei 
AU  - Yu, Huatao 
AU  - Yu, Haibin 
AU  - Liu, Xia 
AU  - Dong, Linlin 

TI  - Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 7
SN  - 1851-5657

AB  - Abscisic acid (ABA) is involved in regulating diverse biological processes, but its signal transduction genes and roles in hemp seed germination are not well known. Here, the ABA signaling pathway members, <i>PYL</i>, <i>PP2C</i> and <i>SnRK2</i> gene families, were identified from the hemp reference genome, including 7 <i>CsPYL</i> (pyrab-actin resistance1-like, ABA receptor), 8 <i>CsPP2CA</i> (group A protein phosphatase 2c), and 7 <i>CsSnRK2</i> (sucrose nonfermenting1-related protein kinase 2). The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage. Exogenous ABA (1 or 10 μM) treatment had a significant regulatory effect on the selected <i>PYL</i>, <i>PP2C</i>, <i>SnRK2</i> gene families. <i>CsAHG3</i> and <i>CsHAI1</i> were most significantly affected by exogenous ABA treatment. Yeast two-hybrid experiments were performed to reveal that CsPYL5, CsSnRK2.2, and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent. Our results indicated that <i>CsPYL5</i>, <i>CsSnRK2.2</i>, <i>CsSnRK2.3</i> and <i>CsPP2CA7</i> might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages. This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination.
KW  - Hemp seeds; abscisic acid; seed germination; <i>PYL-PP2C-SnRK2</i> gene expression

DO  - 10.32604/phyton.2023.029041