TY  - EJOU
AU  - Niu, Minghui 
AU  - Zhang, Heng 
AU  - Li, Xiangyang 
AU  - Hu, Zhibao 
AU  - Zhang, Hongjiao 
AU  - Xu, Zhiru 
AU  - Qu, Chunpu 
AU  - Liu, Guanjun 

TI  - Genome-Wide Analysis of the <i>KANADI</i> Gene Family and Its Expression Patterns under Different Nitrogen Concentrations Treatments in <i>Populus trichocarpa</i>
T2  - Phyton-International Journal of Experimental Botany

PY  - 2023
VL  - 92
IS  - 7
SN  - 1851-5657

AB  - <i>KANADI</i> (<i>KAN</i>) is a plant-specific gene that controlled the polarity development of lateral organs. It mainly acted on the abaxial characteristics of plants to make the lateral organs asymmetrical. However, it had been less identified in woody plants. In this study, the members of the <i>KAN</i> gene family in <i>Populus trichocarpa</i> were identified and analyzed using the bioinformatics method. The results showed that a total of 8 <i>KAN</i> family members were screened out, and each member contained the unique GARP domain and conserved region of the family proteins. Phylogenetic analysis and their gene structures revealed that all <i>KAN</i> genes from <i>P. trichocarpa</i>, <i>Arabidopsis thaliana</i>, and <i>Nicotiana benthamiana</i> could be divided into four subgroups, while the eight genes in <i>P. trichocarpa</i> were classified into three subgroups, respectively. The analysis of tissue-specific expression indicated that <i>PtKAN1</i> was highly expressed in young leaves, <i>PtKAN6</i> was highly expressed in young leaves and mature leaves, <i>PtKAN2</i>, <i>PtKAN5</i>, and <i>PtKAN7</i> were highly expressed in nodes and internodes, <i>PtKAN8</i> was highly expressed in roots, and <i>PtKAN3</i> and <i>PtKAN4</i> showed low expression levels in all tissues. Among them, <i>PtKAN2</i> and <i>PtKAN6</i>, and <i>PtKAN4</i> and <i>PtKAN5</i> might have functional redundancy. Under high nitrogen concentrations, <i>PtKAN2</i> and <i>PtKAN8</i> were highly expressed in mature stems and leaves, respectively, while <i>PtKAN4</i>, <i>PtKAN5</i>, and <i>PtKAN7</i> were highly expressed in roots. This study laid a theoretical foundation for further study of the <i>KAN</i> gene-mediated nitrogen effect on root development.
KW  - Bioinformatics analysis; <i>KANADI</i> gene family; nitrogen; <i>Populus trichocarpa</i>

DO  - 10.32604/phyton.2023.029350