@Article{phyton.2023.027457,
AUTHOR = {Xiaokui Huang, Yingbin Xue, Aaqil Khan, Hanqiao Hu, Naijie Feng, Dianfeng Zheng},
TITLE = {Cloning and Functional Validation of Mung Bean <i>VrPR </i>Gene},
JOURNAL = {Phyton-International Journal of Experimental Botany},
VOLUME = {92},
YEAR = {2023},
NUMBER = {8},
PAGES = {2369--2382},
URL = {http://www.techscience.com/phyton/v92n8/53331},
ISSN = {1851-5657},
ABSTRACT = {For the purpose of functional validation, the mung bean (<i>Vigna radiata</i>) <i>VrPR</i> gene was cloned and overexpressed
in <i>Arabidopsis thaliana</i>. The findings revealed that the ORF of <i>VrPR</i> contained 1200 bp, in which 399 amino acids
were encoded. Bioinformatics analysis showed that the <i>VrPR</i> protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. <i>VrPR</i> was assumed to have 44 amino acid
phosphorylation sites and be contained in chloroplasts. The <i>VrPR</i> secondary structure comprised of random coil,
α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure.
Moreover, analysis of the phylogenetic tree indicated that the soybean PR (Glyma.12G222200) and <i>VrPR</i> were
closely related. Furthermore, chlorophyll content in leaves is markedly increased in Arabidopsis when <i>VrPR</i> is
overexpressed. Our findings will serve as a reference for more functional studies on the PR genes in mung bean.},
DOI = {10.32604/phyton.2023.027457}
}